Mosquito-Associated Isolate Collection (MosAIC)

MosAIC Team at the University of Wisconsin-Madison:

Kerri Coon, Department of Bacteriology
Key personnel at UW-Madison: Holly Nichols, Miguel Medina Muñoz, Jessica Lysne (now at MSU)

 

 

 

Hello! Thank you for your interest in MosAIC—a growing collection of whole genome sequenced bacterial isolates derived from mosquitoes, other dipteran insects, and their habitats. All isolates and genome assemblies associated with the collection are publicly available.

MosAIC partners:

Grant Hughes, Liverpool School of Tropical Medicine
Key personnel at LSTM: Aidan Foo, Laura Brettell, Vishaal Dhokiya, Ananya Hoque

Our Mission

MosAIC is a collaborative project to uncover new insights into mosquito biology, combat vector-borne diseases, and connect researchers across continents worldwide.

We make our isolates and their associated data publicly available for use by educators and researchers to promote the importance of biodiversity science while also serving as a repository for isolates used in basic and applied scientific research.

We seek to inspire and retain students from diverse backgrounds in the sciences through interactive lab- and classroom-based research experiences. Indeed, the first iteration of MosAIC includes >100 isolates contributed by >35 undergraduate Microbiology majors at the University of Wisconsin-Madison.

We are actively expanding the collection to include broader geographic and host species representation, especially from individuals collected from field populations. We hope you’ll consider joining our growing network of researchers!

Interested in contributing?

We accept material both in the form of bacterial isolates or unprocessed tissues. You send us your strains, we’ll store and sequence them for you! Interested in learning more about how to submit? Complete our interest form and we’ll reach out with more information.

MosAIC Interest Form

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Featured Publications

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FAQs

See below

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How do I access MosAIC resources?

We’ve partnered with the American Type Culture Collection (ATCC)* for long-term storage and distribution of MosAIC to the community. Isolates are also available by direct request, especially to researchers in low- and middle-income countries.

*The first iteration of MosAIC is currently being deposited and is expected to become available, in batches, between now and December 2024. In the meantime, please email kerri.coon@wisc.edu with any requests.

Will I be acknowledged for my contributions?

Absolutely! We believe in inclusive research practices that value honesty and transparency. All MosAIC contributors and associated personnel will be offered authorship on relevant scientific papers related to the collection and its expansion at UW-Madison.

Recipients of MosAIC resources agree to acknowledge the MosAIC team and relevant contributors in any publication that contains data or information resulting from the use of MosAIC resources by using the following statement: “This research was made possible with resources from the Mosquito-Associated Isolate Collection (MosAIC), developed in Dr. Kerri Coon’s laboratory at the University of Wisconsin-Madison and available through ATCC (atcc.org). We specifically thank the following MosAIC contributors: <Contributor Name(s)>.”

Recipients will additionally cite MosAIC resources in the following manner:

For isolates:

In text: “<Internal Isolate ID(s)> (e.g., USHLN06), developed as part of the Mosquito-Associated Isolate Collection (MosAIC) in Dr. Kerri Coon’s laboratory at the University of Wisconsin-Madison and available through ATCC (atcc.org).”

For sequencing data:

In text: “<Assembly Accession No.(s)> (e.g., XXX), developed as part of the Mosquito-Associated Isolate Collection (MosAIC) in Dr. Kerri Coon’s laboratory at the University of Wisconsin-Madison and available through the NCBI Sequence Read Archive (ncbi.nlm.nih.gov/sra).”

Primary publication to include in the citation for all isolates and associated sequencing data: “Foo, A., Brettell, L.E., and Nichols, H.L. et al. (2024). Establishment and comparative genomics of a high-quality collection of mosquito-associated bacterial isolates – MosAIC (Mosquito-Associated Isolate Collection).”

Finally, recipients will notify the MosAIC team of such publications by sending an email to kerri.coon@wisc.edu.

Are MosAIC resources Nagoya compliant?

The MosAIC team is committed to honoring the object and purpose of the Nagoya Protocol and is prepared to negotiate in good faith with MosAIC contributors to establish terms that work for them.

All incoming and outgoing transfers of MosAIC materials will be accompanied by a material transfer agreement. The conditions outlined in this agreement will be governed by standard principles for sample access, storage and use, as well as benefit sharing principles such as those outlined in the World Health Organization’s “blueprint” for MTA development. These include:

Equitye.g., Consideration of securing priority status for access to countermeasures developed from the sharing of samples and associated data.

Reciprocity – Access to samples and associated data on the basis of the MTA and the sharing of benefits arising from its use should occur on an equal footing.

Public health interests – Access to benefits should be negotiated in good faith with the consideration of global public health priorities at the core.

Third party access to benefits – Consideration of whether to pass on benefit sharing obligations to third party recipients and successive transfer recipients should be considered and negotiated as appropriate.

More information about the Convention on Biological Diversity and the Nagoya Protocol on Access and Benefit-Sharing can be found HERE.

Thanks to our contributors:

2022 UW-Madison Capstone in Microbiology Students
Doug Brackney, Connecticut Agricultural Experiment Station
Eric Caragata, University of Florida
Marcelo Jacobs-Lorena, Johns Hopkins University
David Lampe, Duquesne University
Michael Povelones, University of Pennsylvania
Sarah Short, Ohio State University
Claire Valiente Moro, University Lyon 1
Jiannong Xu, New Mexico State University

 

Logo design by V. Dhokiya.
Summary figure created with BioRender.com.

Funding support provided by: